400-614-0008

Human CD73 Protein


Cat:CD7-HM173

Description

CD73.png
Synonyms:

CD73 antigen; CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73);  E5NT; EC 3.1.3.5; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase;Purine 5-Prime-Nucleotidase

Formulation and Storage

Formulation:

Supplied as 0.22um filtered solution in  20mM Tris-HCl, 120mM NaCl,4mM CaCl2, 20% Glycerol, pH 7.5.

Storage:

The product should be stored at -70℃. Please do not repeated freeze-thaw cycles.

Assay Data

Tris-Bis PAGE
CD73 gel.png

Recombinant CD73 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

HPLC Data
CD73 HPLC.png

The purity of CD73 (Cat. CD7-HM173) is greater than 95% as determined by SEC-HPLC.

Bioactivity Data 
11.png

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit . The specific activity is >15,000 pmol/min/μg.

ELISA Data
CD73 ELISA.png

Immobilized Human CD73 at 0.5ug/ml(100ul/Well). Dose response curve for Anti-CD73 Ab. with the EC50 of 16.9ng/ml determined by ELISA.

Background

CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A  deficiency of CD73 occurs in a variety of immunodeficiency diseases.

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