Biotinylated Human CD3E/CD3 epsilon 1-27 Protein (CD3-HM2EDB)
Catalog: CD3-HM2EDB
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| Product Description | Recombinant Biotinylated Human CD3E/CD3 epsilon 1-27 Protein is expressed from HEK293 with hFc tag and Avi Tag at the C-Terminus.It contains Asp23-Thr48. |
Product Background |
CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. |
Product Category |
Recombinant Protein |
Target |
CD3E/CD3 epsilon 1-27 |
| Synonyms | CD3e; CD3E; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18 |
Gene/Protein ID |
P07766 |
Species |
Human |
Exact Sequence |
Asp23-Thr48 |
| Molecular Weight | The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E 1-27, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CD3E 1-27, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. |
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The purity of Biotinylated Human CD3E 1-27 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Human CD3E 1-27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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