Mouse LILRB4/CD85k/ILT3 Protein (LIL-MM1B4)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 5.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gly24-Lys238

Catalog: LIL-MM1B4

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Product Description	Recombinant Mouse LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Lys238.
Product Background	LILRB4,also known as CD85k and LIR-5, ILT3,- is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.
Product Category	Recombinant Protein

Target	LILRB4/CD85k/ILT3
Synonyms	HM18; ILT3; ILT-3; LILRB4; LIR5; CD85K
Gene/Protein ID	Q64281-1
Species	Mouse
Exact Sequence	Gly24-Lys238
Molecular Weight	The protein has a predicted MW of 25.1 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 5.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse LILRB4, His Tag immobilized on CM5 Chip can bind Mouse APOE, His Tag with an affinity constant of 5.08 nM as determined in SPR assay (Biacore T200).
	The purity of Mouse LILRB4 is greater than 95% as determined by SEC-HPLC.
	Mouse LILRB4 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.