Currently manufactured according to cGMP guidelines.
Enzymes for every step.
E. Coli Poly(A) Polymerase Tailing Enzyme
Poly(A) polymerase plays a crucial role in mRNA polyadenylation. The enzyme catalyzes the addition of adenosine monophosphates (AMPs) to the 3' end of the mRNA molecule, creating what is known as the poly(A) tail. The poly(A) tail protects RNA from degradation by nucleases, assists in the transport of RNA out of the nucleus, and stimulates translation of the RNA into protein.
Cyclization enzymes are responsible for forming a unique 5'-5' phosphodiester bond between the first and last nucleotides of the mRNA molecule, creating what is known as a circular mRNA. This circularization process is important for increasing the stability and translational efficiency of the mRNA molecule, as well as protecting it from degradation by cellular exonucleases.