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Human 2B4/CD244/SLAMF4 Protein

Product Info
Source

Recombinant Human 2B4/CD244/SLAMF4 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Cys22-Arg221.[Accession | Q9BZW8-2]

Molecular Weight

The protein has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human 2B4/SLAMF4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human 2B4/SLAMF4 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human 2B4 at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.2μg/ml determined by ELISA.

Batch to Batch Consistency

Immobilized Human 2B4 at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.2/0.3/0.2μg/ml determined by ELISA.

Background

2B4 (CD244) is expressed by memory-phenotype CD8( ) T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.

Synonyms

2B4; CD244 antigen; CD244 molecule, natural killer cell receptor 2B4; CD244; h2B4; NAIL; NAILNK cell activation-inducing ligand; natural killer cell receptor 2B4; NKR2B4; NKR2B4NK cell type I receptor protein 2B4,2B4CD244 natural killer cell receptor 2B4; Nmrk; SLAMF4; SLAMF4NK cell activation inducing ligand NAIL

References

(1)Velikovsky C A , Deng L , Chlewicki L K , et al. Structure of Natural Killer Receptor 2B4 Bound to CD48 Reveals Basis for Heterophilic Recognition in Signaling Lymphocyte Activation Molecule Family[J]. Immunity, 2007, 27(4):0-584.

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