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Human ACE2/ACEH Protein

Product Info
Source

Recombinant Human ACE2/ACEH Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gln18-Ser740.[Accession | Q9BYF1]

Molecular Weight

The protein has a predicted MW of 84.7 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Formulation

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

Storage

The product should be stored at -70℃. Please do not repeated freeze-thaw cycles.

Assay Data
Tris-Bis PAGE

Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human ACE2 is greater than 95% as determined by SEC-HPLC

SPR Data

SARS-COV-2 Spike RBD captured on Protein A chip, can bind Human ACE2, His Tag with an affinity constant of 11.9nM as determined in a SPR assay (Biacore T200).

Background

ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.

Synonyms

ACE-2;ACEH;ACE2

References

(1)Kuba K , Imai Y , Rao S , et al. A crucial role of angiotensin converting enzyme 2 (ACE2) in SARS coronavirus–induced lung injury[J]. Nature Medicine, 2005, 11(8):875-879.

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