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Human ANGPTL3/Angiopoietin-like 3 Protein

Product Info
Source

Recombinant Human ANGPTL3/Angiopoietin-like 3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ser17-Lys219.[Accession | Q9Y5C1]

Molecular Weight

The protein has a predicted MW of 26.6 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE

Formulation

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

Storage

The product should be stored at -70℃. Please do not repeated freeze-thaw cycles.

Assay Data
Tris-Bis PAGE

Human Angiopoietin-like 3 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

ELISA Data

Immobilized Human Angiopoietin-like 3,His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-ANGPTL3 Ab.hFc Tag with the EC50 of 12.2ng/ml determined by ELISA.

SPR Data

Human LILRB2 captured on Protein A chip, can bind Human ANGPTL3, His Tag with an affinity constant of 17.6nM as determined in a SPR assay (Biacore T200).

Background

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake .

Synonyms

AGNPT5; Ang-5; angiopoietin 5; Angiopoietin-5; angiopoietin-like 3; Angiopoietin-like Protein 3; angiopoietin-related protein 3; ANGPT5angiopoietin-5; ANGPTL3; FHBL2

References

(1) Oike Y , Akao M , Kubota Y , et al. Angiopoietin-like proteins: potential new targets for metabolic syndrome therapy[J]. Trends in Molecular Medicine, 2005, 11(10):473-479.

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