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Human 4-1BB/TNFRSF9 Protein

Product Info
Source

Recombinant Human 4-1BB/TNFRSF9 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Leu24-Gln186.[Accession | Q07011]

Molecular Weight

The protein has a predicted MW of 18.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human 4-1BB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human 4-1BB is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human 4-1BB, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human 4-1BBL, hFc Tag with the EC50 of 0.24μg/ml determined by ELISA.

Background

4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation.

Synonyms

CD137 antigen; CD137;TNFRSF9; 4-1BB ligand receptor; 41BB; 4-1BB; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9

References

(1)Vinay D S , Kwon B S . 4-1BB (CD137), an inducible costimulatory receptor, as a specific target for cancer therapy[J]. BMB Reports, 2014, 47(3):122-129.

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