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Human B7-H3/CD276 Protein

Product Info
Source

Recombinant Human B7-H3/CD276 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Leu29-Pro245.[Accession | Q5ZPR3-2]

Molecular Weight

The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human B7-H3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human B7-H3 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human B7-H3 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Human B7-H3 Ab., hFc Tag with the EC50 of 24.8ng/ml determined by ELISA.

Background

B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma ) production, and cytotoxic T cells induction.

Synonyms

B7H3; B7-H3; CD276 antigen; CD276 molecule; CD276; B7H34Ig-B7-H3; B7-H3B7 homolog 3; Costimulatory molecule

References

(1)Fodstad O , Tekle C , Chen Y W . The Immunoregulatory Protein Human B7H3 is a Tumor-Associated Antigen that Regulates Tumor Cell Migration and Invasion[J]. Current Cancer Drug Targets, 2008, 8(5)

(2)Du H, Hirabayashi K, Ahn S, et al. Antitumor Responses in the Absence of Toxicity in Solid Tumors by Targeting B7-H3 via Chimeric Antigen Receptor T Cells[J]. Cancer Cell, 2019, 35(2).

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