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Human B7-H4 Protein

Product Info
Source

Recombinant Human B7-H4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Phe29-Ala258.[Accession | Q7Z7D3]

Molecular Weight

The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 42-50 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human B7-H4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human B7-H4 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human B7-H4,His tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-B7-H4 Ab.,hFc Tag with the EC50 of 10.46ng/ml determined by ELISA.

Background

B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis . A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status .

Synonyms

B7H4; B7-H4; B7H4 T-cell costimulatory molecule B7x; B7S1; B7h.5; B7S1VCTN1; B7x; B7XPRO1291; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; Vtcn1

References

(1)Smith J B, Caitlin S, Powell D J. B7-H4 as a potential target for immunotherapy for gynecologic cancers: a closer look[J]. Gynecologic Oncology, 2014, 134(1):181-189.

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