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Human BTLA Protein

Product Info
Source

Recombinant Human BTLA Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Lys31-Ser150.[Accession | Q7Z6A9]

Molecular Weight

The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human BTLA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human BTLA is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human BTLA at 0.5μg/ml (100μl/Well). Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA.

Batch to Batch Consistency

Immobilized Human BTLA at 0.5μg/ml (100μl/Well). Dose response curve for Anti-BTLA Antiody, hFc Tag with the EC50 of 15.7 / 17.8 / 16.7ng/ml determined by ELISA.

SPR Data

Anti-BTLA antibody captured on Protein A chip, can bind Human BTLA, His Tag with an affinity constant of 0.99nM as determined in a SPR assay (Biacore T200).

Background

B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 . BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.

Synonyms

CD272 antigen; CD272; B and T lymphocyte associated; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA; BTLA1; FLJ16065; MGC129743

References

(1)Haymaker C L , Wu R C , Ritthipichai K , et al. BTLA marks a less-differentiated tumor-infiltrating lymphocyte subset in melanoma with enhanced survival properties[J]. OncoImmunology, 2015, 4(8):e1014246.

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