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Human EpCAM/TROP1 Protein

Product Info
Source

Recombinant Human EpCAM/TROP1 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gln24-Lys245 .[Accession | P16422]

Molecular Weight

The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

Storage

The product should be stored at -70℃. Please do not repeated freeze-thaw cycles.

Assay Data
Tris-Bis PAGE

Human EpCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..

SEC-HPLC

The purity of Human EpCAM is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human EpCAM,His Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-EpCAM Ab.,hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.

Background

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.

Synonyms

CD326 antigen; CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen;17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; ;Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A

References

(1)Pak M G , Shin D H , Lee C H , et al. Significance of EpCAM and TROP2 expression in non-small cell lung cancer[J]. World Journal of Surgical Oncology, 2012, 10(1):53

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