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Human CEACAM-5/CD66e Protein

Product Info
Source

Recombinant Human CEACAM-5/CD66e Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Lys35-Ala685.[Accession | Q8N4D0]

Molecular Weight

The protein has a predicted MW of 74.2 kDa. Due to glycosylation, the protein migrates to 115-150 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human CEACAM-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human CEACAM-5 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human CEACAM-5 at 0.5μg/ml (100μl/well). Dose response curve for Anti-CEACAM Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA.

SPR Data

Human CEACAM-5, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-CEACAM5 Antibody (labetuzumab), hFc Tag with an affinity constant of 0.12nM as determined in SPR assay (Biacore T200).

Background

Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver. CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors, and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated.

Synonyms

CEACAM-5、CD66e、CEA、Meconium antigen 100

References

(1)Li Q, Li Y, Li J, et al. FBW7 suppresses metastasis of colorectal cancer by inhibiting HIF1α/CEACAM5 functional axis. Int J Biol Sci. 2018;14(7):726-735. Published 2018 May 12. doi:10.7150/ijbs.24505

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