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Human CD24 Protein

Product Info
Source

Recombinant Human CD24 Protein is expressed from Expi293 with mFc tag at the C-terminal. It contains Ser27-Gly59.[Accession | P25063]

Molecular Weight

The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human CD24 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC

The purity of Human CD24 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human CD24 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD24 Antibody, hFc Tag with the EC50 of 0.19μg/ml determined by ELISA.

Background

CD24 is a sialoglycoprotein expressed at the surface of most B lymphocytes and differentiating neuroblasts. It is also expressed on neutrophils and neutrophil precursors from the myelocyte stage onwards. The potential for targeting CD24 in cancer therapy seems promising, as CD24 is overexpressed in many human cancers.

Synonyms

CD 24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24; CD24A; CD24Asignal transducer CD24; FLJ22950; FLJ43543; MGC75043; Small cell lung carcinoma cluster 4 antigen

References

(1)Barkal AA, et al.CD24 signalling through macrophage Siglec-10 is a target for cancer immunotherapy.Nature. 2019 Jul 31. doi: 10.1038/s41586-019-1456-0.

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