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Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein

Product Info
Source

Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from Expi293 with His tag at the C-terminal. It contains Asp23-Glu120 (CD3E)&Phe22-Ala105 (CD3D).[Accession | P07766&P04234]

Molecular Weight

The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human CD3E&CD3D at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 38ng/ml determined by ELISA.

SPR Data

Human CD3E&CD3D, His Tag can bind Anti-Human CD3 Antibody, Mouse IgG2a with an affinity constant of 0.36nM as determined in SPR assay (Biacore T200).

Background

T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Synonyms

CD3;T-cell surface glycoprotein CD3 epsilon chain;CD3e;CD3E;T-cell surface glycoprotein CD3 delta chain;T-cell receptor T3 delta chain;CD3d;T3D;CD3D

References

(1)Penaranda C , Tang Q , Bluestone J A . Anti-CD3 Therapy Promotes Tolerance by Selectively Depleting Pathogenic Cells while Preserving Regulatory T Cells[J]. The Journal of Immunology, 2011, 187(4):2015-2022.

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