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Human CD38 Protein

Product Info
Source

Recombinant Human CD38 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Val43-Ile300.[Accession | P28907]

Molecular Weight

The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 45-58 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human CD38 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human CD38 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human CD38 at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 28ng/ml determined by ELISA.

SPR Data

Anti-CD38 Antibody captured on Protein A chip, can bind Human CD38, His Tag with an affinity constant of 0.23μM as determined in a SPR assay (Biacore T200).

Background

CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase, is a transmembrane glycoprotein found on the surface of some immune cells including plasma cells, activated or immature T and B cells, monocytes, and natural killer cells. CD38 participates in cell adhesion, signal transduction and calcium signaling.

Synonyms

CD38 antigen; CD38 molecule; CD38; Cyclic ADP-ribose hydrolase 1; ADP-ribosyl cyclase 1; ADP-ribosyl Cyclase; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase; cADPr hydrolase 1; CD38 antigen (p45); Cyclic ADP-ribose Hydrolase; EC 3.2.2.5; NAD( ) nucleosidase; T10

References

(1)Alba MatasCéspedes, Vidalcrespo A , Rodriguez V , et al. Daratumumab, a Novel Human Anti-CD38 Monoclonal Antibody for the Treatment of Chronic Lymphocytic Leukemia and B-Cell Non–Hodgkin Lymphoma[J]. Blood, 2012.

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