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Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein

Product Info
Source

Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from Expi293 with hFc at the C-terminal. It contains Asp23-Asp126(CD3E)&Phe22-Ala105(CD3D).[Accession | P07766&P04234]

Molecular Weight

The protein has a predicted MW of 37.8 kDa (CD3E) and 35.4 kDa (CD3D). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human CD3E&CD3D was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human CD3E&CD3D at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1, hFc Tag with the EC50 of 31.68ng/ml determined by ELISA.

ELISA Data

Immobilized Human CD3E&CD3D at 0.1ug/ml (100ul/Well). Dose response curve for Anti-CD3E&CD3D Ab.2, mFc Tag with the EC50 of 27.0ng/ml determined by ELISA.

Batch to Batch Consistency

Immobilized Human CD3E&CD3D at 1μg/ml (100μl/Well). Dose response curve for Anti-CD3E&CD3D Ab., mFc Tag (OKT3) with the EC50 of 28.8/25.5/27.8ng/ml determined by ELISA.

37℃ Stability Test

Immobilized Human CD3E&CD3D at 1μg/ml (100μl/Well). Dose response curve for Anti-CD3E&CD3D Ab., mFc Tag (OKT3) with the EC50 of 23.8/22.6/23.5/22.8ng/ml determined by ELISA.

Background

T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Synonyms

CD3;T-cell surface glycoprotein CD3 epsilon chain;CD3e;CD3E;T-cell surface glycoprotein CD3 delta chain;T-cell receptor T3 delta chain;CD3d;T3D;CD3D

References

(1)Penaranda C , Tang Q , Bluestone J A . Anti-CD3 Therapy Promotes Tolerance by Selectively Depleting Pathogenic Cells while Preserving Regulatory T Cells[J]. The Journal of Immunology, 2011, 187(4):2015-2022.

(2)Gedeon P C , Schaller T H , Chitneni S K , et al. A rationally designed fully human EGFRvIII:CD3-targeted bispecific antibody redirects human T cells to treat patient-derived intracerebral malignant glioma.[J]. Clinical Cancer Research, 2018:clincanres.0126.2017.

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