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Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein

Product Info
Source

Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Asp23-Asp126(CD3E)&Gln23-Ser116(CD3G).[Accession | P07766&P09693]

Molecular Weight

The protein has a predicted MW of 37.8 kDa (CD3E) and 36.5 kDa (CD3G).Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human CD3E&CD3G/CD3 epsilon&CD3 gamma on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human CD3E&CD3G/CD3 Epsilon&CD3 Gamma was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human CD3E&CD3G at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3G Ab.1, hFc Tag with the EC50 of 1.22ug/ml determined by ELISA.

ELISA Data

Immobilized Human CD3E&CD3G at 1μg/ml (100μl/Well). Dose response curve for Anti-CD3E&CD3G Ab.2, mFc Tag with the EC50 of 20.7ng/ml determined by ELISA.

Batch to Batch Consistency

Immobilized Human CD3E&CD3G at 1μg/ml (100μl/Well). Dose response curve for Anti-CD3E&CD3G Ab.(OKT3), mFc Tag with the EC50 of 20.7/21.0/20.0ng/ml determined by ELISA.

37℃ Stability Test

Immobilized Human CD3E&CD3G at 1μg/ml (100μl/Well). Dose response curve for Anti-CD3E&CD3G Ab.(OKT3), mFc Tag with the EC50 of 20.7/19.5/20.0/20.7ng/ml determined by ELISA.

Background

T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Synonyms

CD3;CD3G;T-cell surface glycoprotein CD3 gamma chain;T-cell receptor T3 gamma chain;CD3g;T3G;T-cell surface glycoprotein CD3 epsilon chain;CD3e;CD3E

References

(1)Penaranda C , Tang Q , Bluestone J A . Anti-CD3 Therapy Promotes Tolerance by Selectively Depleting Pathogenic Cells while Preserving Regulatory T Cells[J]. The Journal of Immunology, 2011, 187(4):2015-2022.

(2)Gedeon P C , Schaller T H , Chitneni S K , et al. A rationally designed fully human EGFRvIII:CD3-targeted bispecific antibody redirects human T cells to treat patient-derived intracerebral malignant glioma.[J]. Clinical Cancer Research, 2018:clincanres.0126.2017.

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