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Human CD30/TNFRSF8 Protein

Product Info
Source

Recombinant Human CD30/TNFRSF8 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Phe19-Lys379.[Accession | P28908]

Molecular Weight

The protein has a predicted MW of 41.3 kDa. Due to glycosylation, the protein migrates to 68-95 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human CD30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human CD30 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human CD30 at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 0.16μg/ml determined by ELISA.

Background

Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.Most specific Hodgkin disease associated antigen.

Synonyms

CD30 antigen; CD30; CD30KI-1; CD30L receptor; TNFRSF8; cytokine receptor CD30; D1S166EKi-1; Ki-1 antigen; Lymphocyte activation antigen CD30; tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8

References

(1)Van d W C A , Pileri S A , Feldman A L , et al. Understanding CD30 biology and therapeutic targeting: a historical perspective providing insight into future directions[J]. Blood Cancer Journal, 2017, 7(9):e603.

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