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Human Siglec-3/CD33 Protein

Product Info
Source

Recombinant Human Siglec-3/CD33 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Asp18-His259 .[Accession | P20138]

Molecular Weight

The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

Storage

The product should be stored at -70℃. Please do not repeated freeze-thaw cycles.

Assay Data
Tris-Bis PAGE

Human Siglec-3/CD33 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Siglec-3/CD33 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human Siglec-3, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-Siglec3 Ab., hFc Tag with the EC50 of 19.9ng/ml determined by ELISA.

Background

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11 . To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.

Synonyms

CD33 antigen (gp67); CD33 antigen; CD33 molecule; CD33; FLJ00391; gp67; myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec3; Siglec-3; SIGLEC3gp67

References

(1)Walter R B , Appelbaum F R , Estey E H , et al. Acute myeloid leukemia stem cells and CD33-targeted immunotherapy[J]. Blood, 2012, 119(26):6198-6208.

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