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Human CD47 Protein

Product Info
Source

Recombinant Human CD47 is expressed from Expi293 with His tag at the C-terminal. It contains Gln19-Pro139.[Accession | Q08722]

Molecular Weight

The protein has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Human CD47 at 5μg/ml (100μl/Well). Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 95.0ng/ml determined by ELISA.

Batch to Batch Consistency

Immobilized Human CD47 at 5μg/ml (100μl/Well). Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 95.0/94.7/93.8ng/ml determined by ELISA.

SPR Data

Human CD47, His Tag captured on CM5 Chip via anti-His antibody can bind Human Sirpa, hFc Tag with an affinity constant of 18.9nM as determined in SPR assay (Biacore T200).

Background

CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.

Synonyms

CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47; antigen identified by monoclonal 1D8; Antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer);IAP; IAPintegrin associated protein; Integrin-associated protein; leukocyte surface antigen CD47; MER6integrin-associated signal transducer; OA3; Protein MER6; Rh-related antigen

References

(1)Weiskopf K , Ring A M , Ho C C M , et al. Engineered SIRP? Variants as Immunotherapeutic Adjuvants to Anticancer Antibodies[J]. Science, 2013, 341(6141):88-91.

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