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Human CD7 Protein

Product Info
Source

Recombinant Human CD7 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ala26-Pro180.[Accession | P09564]

Molecular Weight

The protein has a predicted MW of 19.3 kDa. Due to glycosylation, the protein migrates to 34-48 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human CD7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human CD7 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human CD7 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD7 Antibody, hFc Tag with the EC50 7.9ng/ml determined by ELISA.

Background

CD7, also known as Leu-9, is an approximately 40 kDa glycosylated and palmitoylated transmembrane protein in the immunoglobulin superfamily.CD7 is expressed on T cells, NK cells , myeloid progenitor cells, and CD19 B progenitor cells . Among CD8 T cells, the CD7-bright population preferentially contains naïve and memory cells, while more weak expressors are primarily effector cells.

Synonyms

CD7 antigen (p41); CD7 antigen; CD7 molecule; CD7; GP40T-cell surface antigen Leu-9; LEU-9; T-cell antigen CD7; T-cell leukemia antigen; Tp40; TP41; TP41p41 protein

References

(1)Gomes-Silva D , Srinivasan M , Sharma S , et al. CD7-edited T cells expressing a CD7-specific CAR for the therapy of T-cell malignancies[J]. Blood, 2017:blood-2017-01-761320.

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