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Human/Cynomolgus/Rhesus macaque CD28 Protein

Product Info
Source

Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Asn19-Pro152.[Accession | P10747]

Molecular Weight

The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human/Cynomolgus/Rhesus macaque CD28 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human/Cynomolgus/Rhesus macaque CD28 was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human/Cynomolgus/Rhesus macaque CD28 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA.

SPR Data

Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15nM as determined in a SPR assay (Biacore T200).

Background

CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Synonyms

CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44

References

(1)Suntharalingam G , Perry M R , Ward S , et al. Cytokine Storm in a Phase 1 Trial of the Anti-CD28 Monoclonal Antibody TGN1412[J]. New England Journal of Medicine, 2006, 355(10):1018-1028.

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