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Cynomolgus CD3E/CD3 epsilon Protein

Product Info
Source

Recombinant Cynomolgus CD3E/CD3 epsilon Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Gln22-Asp117.[Accession | Q95LI5-1]

Molecular Weight

The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Cynomolgus CD3E on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Cynomolgus CD3E Protein is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Cynomolgus CD3E at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E Ab., hFc Tag with the EC50 of 0.2μg/ml determined by ELISA.

Background

CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Synonyms

T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e;CD3E;T3E

References

(1)Karasu Ebru E, Nilsson Bo B, Köhl Jörg J, Lambris John D JD

(2)Front Immunol 2019-05-03 994 10

(3)Zelek Wioleta M WM, Taylor Philip R PR

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