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Human CD3E/CD3 epsilon Protein

Product Info
Source

Recombinant Human CD3E/CD3 epsilon Protein is expressed from Expi293 with His tag at the C-terminal. It contains Asp23-Asp126.[Accession | P07766]

Molecular Weight

The protein has a predicted MW of 12.6 kDa. Due to glycosylation, the protein migrates to 15 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human CD3 epsilon on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Human CD3 epsilon at 1μg/ml (100μl/Well). Dose response curve for Anti-CD3 Antibody, hFc Tag with the EC50 of 0.2μg/ml determined by ELISA.

Background

CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.

Synonyms

T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;CD3e;CD3E;T3E

References

(1)Kroesen B J , Nieken J , Sleijfer D T , et al. Approaches to lung cancer treatment using the CD3E×GP-2-directed Bispecific Monoclonal Antibody BIS-1[J]. Cancer Immunology Immunotherapy, 1997, 45(3-4):203-206..

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