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SARS-COV-2 Spike S1 (N501Y) Protein

Product Info
Source

Recombinant SARS-COV-2 Spike S1 (N501Y) Protein is expressed from Expi293 with His tag at the C-terminal. It contains Val16-Arg685.[Accession | YP_009724390.1]

Molecular Weight

The protein has a predicted MW of 76 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

SARS-COV-2 Spike S1 (N501Y) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of SARS-COV-2 Spike S1 (N501Y) is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized SARS-COV-2 Spike S1 (N501Y) at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2 , hFc Tag with the EC50 of 80.3ng/ml determined by ELISA.

Background

A new variant of SARS-CoV-2 is spreading in the UK and is rapidly becoming a global threat.It is characterised by multiple mutations in the spike protein. Among them, N501Y is of major concern because it involves one of the six key amino acid residues determining a tight interaction of the SARS-CoV-2 receptor-binding domain (RBD) with its cellular receptor angiotensin-converting enzyme 2 (ACE2).

Synonyms

S1 protein;Spike glycoprotein Subunit1;S glycoprotein Subunit1;Spike protein S1

References

(1)Kemp SA, Collier DA, Datir R, Ferreira I, Gayed S, Jahun A, Hosmillo M, Rees-Spear C, Mlcochova P, Lumb IU, Roberts DJ, Chandra A, Temperton N, Sharrocks K, Blane E, Briggs J, van Gils MJ, Smith K, Bradley JR, Smith C, Doffinger R, Ceron-Gutierrez L, Barcenas-Morales G, Pollock DD, Goldstein RA, Smielewska A, Skittrall JP, Gouliouris T, Goodfellow IG, Gkrania-Klotsas E, Illingworth C, McCoy LE, Gupta RK. Neutralising antibodies in Spike mediated SARS-CoV-2 adaptation. medRxiv [Preprint]. 2020 Dec 29:2020.12.05.20241927. doi: 10.1101/2020.12.05.20241927. PMID: 33398302; PMCID: PMC7781345.

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