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SARS-COV-2 Spike S1 Protein

Product Info
Source

Recombinant SARS-COV-2 Spike S1 Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Val16-Arg685.[Accession | QHO60594.1]

Molecular Weight

The protein has a predicted MW of 101.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Formulation

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

Storage

The product should be stored at -70℃. Please do not repeated freeze-thaw cycles.

Assay Data
Tris-Bis PAGE

SARS-COV-2 Spike S1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of SARS-COV-2 Spike S1 is greater than 95% as determined by SEC-HPLC.

Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Synonyms

S1 protein;Spike glycoprotein Subunit1;S glycoprotein Subunit1;Spike protein S1

References

(1)Ren W, Sun H, Gao GF, Chen J, Sun S, Zhao R, Gao G, Hu Y, Zhao G, Chen Y, Jin X, Fang F, Chen J, Wang Q, Gong S, Gao W, Sun Y, Su J, He A, Cheng X, Li M, Xia C, Li M, Sun L. Recombinant SARS-CoV-2 spike S1-Fc fusion protein induced high levels of neutralizing responses in nonhuman primates. Vaccine. 2020 Jul 31;38(35):5653-5658. doi: 10.1016/j.vaccine.2020.06.066. Epub 2020 Jun 24. PMID: 32651113; PMCID: PMC7311893.

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