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SARS-COV-2 Spike S1 (D614G) Protein

Product Info
Source

Recombinant SARS-COV-2 Spike S1 (D614G) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln14-Arg683(D614G).[Accession | QHD43416.1]

Molecular Weight

The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

SARS-COV-2 Spike S1 (D614G) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of SARS-COV-2 Spike S1 (D614G) is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized SARS-CoV-2 S1 (D614G), His Tag at 2μg/ml (100μl/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 42ng/ml determined by ELISA.

Background

The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.

Synonyms

S1 protein;Spike glycoprotein Subunit1;S glycoprotein Subunit1;Spike protein S1

References

(1)Zhang L, Jackson CB, Mou H, Ojha A, Rangarajan ES, Izard T, Farzan M, Choe H. The D614G mutation in the SARS-CoV-2 spike protein reduces S1 shedding and increases infectivity. bioRxiv [Preprint]. 2020 Jun 12:2020.06.12.148726. doi: 10.1101/2020.06.12.148726. Update in: Nat Commun. 2020 Nov 26;11(1):6013. PMID: 32587973; PMCID: PMC7310631.

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