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SARS-COV-2 Spike S Trimer Protein

Product Info
Source

Recombinant SARS-COV-2 Spike S is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Val16-Glu1188.[Accession | QHD43416.1]

Molecular Weight

The protein has a predicted MW of 136.6 kDa. Due to glycosylation, the protein migrates to 175-230 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

SARS-COV-2 Spike S Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of SARS-COV-2 Spike S Trimer is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized SARS-COV-2 Spike S Trimer at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 37.8ng/ml determined by ELISA.

Batch to Batch Consistency

Immobilized SARS-COV-2 Spike S Trimer at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 47.3/37.8/37.0ng/ml determined by ELISA.

Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Synonyms

S protein,Spike glycoprotein,S glycoprotein,COVID-19

References

(1)Parsons, Lisa, Bouwman, Kim M, Azurmendi, Hugo F. Glycosylation of the viral attachment protein of avian coronavirus is essential for host cell and receptor binding[J]. Journal of Biological Chemistry.

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