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Human EGFR/HER1 Protein

Product Info
Source

Recombinant Human EGFR/HER1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu25-Ser645.[Accession | P00533]

Molecular Weight

The protein has a predicted MW of 71.5 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human EGFR/HER1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of  Human EGFR/HER1 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human EGFR at 0.5μg/ml (100μl/Well). Dose response curve for Anti-EGFR Antibody, hFc Tag with the EC50 of 0.34μg/ml determined by ELISA.

Background

The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.

Synonyms

ErbB; ErbB1; HER1; HER-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ERBB1PIG61;mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

References

(1)Seshacharyulu P , Ponnusamy M P , Haridas D , et al. Targeting the EGFR signaling pathway in cancer therapy[J]. Expert Opinion on Therapeutic Targets, 2012.

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