Bulk Quote Inquiry

FAP-HM101-100μg / Inquiry

FAP-HM101-500μg / Inquiry

FAP-HM101-1mg / Inquiry

Human FAP Protein

Product Info
Source

Recombinant Human FAP Protein is expressed from Expi293 with His tag at the N-terminal. It contains Leu26-Asp760.[Accession | Q12884]

Molecular Weight

The protein has a predicted MW of 86.1 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in 20mM Tris, 250mM NaCl (pH 8.2). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human FAP on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC

The purity of Human FAP is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human FAP,His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FAP Ab., hFc Tag with the EC50 of 25.8ng/ml determined by ELISA.

Background

Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.

Synonyms

FAP;FAPalpha;SIMP;Seprase;APCE

References

(1)Zou B, Liu X, Zhang B, et al. The Expression of FAP in Hepatocellular Carcinoma Cells is Induced by Hypoxia and Correlates with Poor Clinical Outcomes. J Cancer. 2018;9(18):3278-3286. Published 2018 Sep 7. doi:10.7150/jca.25775.

Kactus Biosystems Co.Ltd
Online Message