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Human FGF21 Protein

Product Info
Source

Recombinant Human FGF21 Protein is expressed from Expi293 with mFc tag and Avi tag at the N-terminal. It contains His29-Ser209.[Accession | Q9NSA1-1]

Molecular Weight

The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human FGF21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human FGF21 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human FGF21 at 2μg/ml (100μl/Well) on the plate. Dose response curve for Full Length Human Klotho, His Tag with the EC50 of 1.48μg/ml determined by ELISA.

SPR Data

Human Klotho full length, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).

Background

Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.

Synonyms

UNQ3115 、 PRO10196

References

(1) Lewis JE, Ebling FJP, Samms RJ, Tsintzas K. Going Back to the Biology of FGF21: New Insights. Trends Endocrinol Metab. 2019;30(8):491-504. doi:10.1016/j.tem.2019.05.007

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