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Human FOLR1 Protein

Product Info
Source

Recombinant Human FOLR1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Arg25-Met233 .[Accession | P15328]

Molecular Weight

The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

Storage

The product should be stored at -70℃. Please do not repeated freeze-thaw cycles.

Assay Data
Tris-Bis PAGE

Human FOLR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human FOLR1 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human FOLR1 at 0.2μg/ml (100μl/Well) on plate.Dose response curve for Anti-FOLR1 Ab., hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.

Background

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH.

Synonyms

FOLR; FOLR1; FR-alpha;Adult folate-binding protein; FBP; folate binding protein; folate receptor 1 (adult); Folate receptor 1; folate receptor alpha; Folate receptor, adult; Folbp1; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18

References

(1)Anthony C , Bax H J , Josephs D H , et al. Targeting folate receptor alpha for cancer treatment[J]. Oncotarget, 2016, 7(32).

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