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Human GITR/TNFRSF18 Protein

Product Info
Source

Recombinant Human GITR/TNFRSF18 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Gln26-Glu161.[Accession | Q9Y5U5]

Molecular Weight

The protein has a predicted MW of 15.4 kDa. Due to glycosylation, the protein migrates to 25-28 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human GITR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%

SEC-HPLC

The purity of Human GITR is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human GITR at 1μg/ml (100μl/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 0.2μg/ml determined by ELISA.

ELISA Data

Immobilized Human GITR at 0.5μg (100μl/Well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.

Batch to Batch Consistency

Immobilized Human GITR at 0.5μg (100μl/Well) on the plate. Dose response curve for Anti-GITR Antibody, hFc Tag with the EC50 of 7.0/9.0/7.0ng/ml determined by ELISA.

Background

GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Synonyms

CD357 antigen; CD357; GITR; GITR-D; TNFRSF18; Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; tumor necrosis factor receptor superfamily, member 181

References

(1)Knee D A , Hewes B , Brogdon J L . Rationale for anti-GITR cancer immunotherapy[J]. European Journal of Cancer, 2016, 67:1-10.

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