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Human Her3/ErbB3 Protein

Product Info
Source

Recombinant Human Her3/ErbB3 is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Ser20-Thr643.[Accession | P21860]

Molecular Weight

The protein has a predicted MW of 71.6 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human Her3/ErbB3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Her3/ErbB3 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human Her3 at 1μg/ml (100ul/Well). Dose response curve for Anti-Her3 Ab., hFc Tag with the EC50 of 0.2μg/ml determined by ELISA.

Background

Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).

Synonyms

ErbB3; ErbB-3; erbB3-S; HER3; HER3c-erbB-3;c-erbB3; EC 2.7.10; EC 2.7.10.1; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

References

(1)Yonesaka K , Haratani K , Hirotani K , et al. Abstract 44: U3-1402, a novel HER3-targeting ADC, and a novel DNA topoisomerase I inhibitor inhibit the growth of non-small cell lung cancer with EGFR mutation[J]. Cancer Research, 2017, 77(13 Supplement):44-44.

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