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Full Length Human Beta Klotho Protein

Product Info
Source

Recombinant Full Length Human Beta Klotho Protein is expressed from Expi293 with His tag at the C-terminal. It contains Met30-Thr983.[Accession | Q86Z14]

Molecular Weight

The protein has a predicted MW of 111.6 kDa. Due to glycosylation, the protein migrates to 115-125 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human Beta Klotho on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC

The purity of Human Beta Klotho is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Full Length Human Klotho, His Tag at 2μg/ml (100μl/Well). Dose response curve for Anti-Klotho Ab.1,hFc Tag with the EC50 of 38.5ng/ml determined by ELISA.

ELISA Data

Immobilized Full Length Human Klotho, His Tag at 2μg/ml (100μl/Well).Dose response curve for Anti-Klotho Ab. 2,hFc Tag with the EC50 of 18.9ng/ml determined by ELISA.

SPR Data

Human Klotho full length, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).

Background

Klotho is an anti-aging single-pass membrane protein predominantly produced in the kidney, with shedding of the amino-terminal extracellular domain into the systemic circulation. Circulating levels of soluble Klotho decrease with age, and the klotho gene is associated with increased risk of age-related diseases.

Synonyms

betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; klotho beta-like protein; MGC142213

References

(1)Lin Y , Sun Z . Antiaging Gene Klotho Attenuates Pancreatic β-Cell Apoptosis in Type 1 Diabetes[J]. Diabetes, 2015, 64(12):4298-4311.

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