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Human LILRA2/CD85h/ILT1 Protein

Product Info
Source

Recombinant Human LILRA2/CD85h/ILT1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly24-Asn449.[Accession | Q8N149]

Molecular Weight

The protein has a predicted MW of 49.9 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human LILRA2 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC

The purity of Human LILRA2/CD85h/ILT1 Protein is greater than 95% as determined by SEC-HPLC.

Background

LILRA2, also known as ILT1, CD85h, and LIR7, is an approximately 70 kDa variably glycosylated transmembrane protein that regulates immune cell activation. Mature human LILRA2 consists of a 426 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail. LILRA2 is part of the innate immune responses against microbial infection.

Synonyms

CD85 antigen-like family member H; CD85h antigen; CD85h; ILT1; ILT-1; ILT1CD85H; Leukocyte immunoglobulin-like receptor 7; leukocyte immunoglobulin-like receptor subfamily A member 2 soluble; leukocyte immunoglobulin-like receptor subfamily A member 2; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2; LILRA2; LIR7; LIR7LIR-7Immunoglobulin-like transcript 1

References

(1)Lu H K , Ainslie M , Yasumi E , et al. LILRA2 Selectively Modulates LPS-Mediated Cytokine Production and Inhibits Phagocytosis by Monocytes[J]. PLoS ONE, 2012, 7(3):e33478-.

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