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Human LILRA3/CD85e Protein

Product Info
Source

Recombinant Human LILRA3/CD85e Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly24-Glu439.[Accession | Q8N6C8]

Molecular Weight

The protein has a predicted MW of 47.9 kDa. Due to glycosylation, the protein migrates to 68-72 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human LILRA3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human LILRA3 is greater than 95% as determined by SEC-HPLC.

Background

Human LILRA3, also known as Leukocyte Ig-like receptor 4 (LIR-4), CD85 antigen-like family member E, Immunogobulin-like transcript 6 (ILT-6) and monocyte inhibitory receptor HM43/HM31,  is a 70 kD Ig superfamily member that belongs to the leukocyte receptor complex/cluster. Mature LILRA3 is 416 amino acids (aa) in length and contains four C2-type Ig-like domains . Unlike other LILR family members, LILRA3 is actively secreted.LILRA3 acts as soluble receptor for class I MHC antigens. LILRA3 binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. Binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes.

Synonyms

CD85 antigen-like family member E; CD85e antigen; CD85e; HM31; HM43; ILT6; ILT-6; ILT6CD85e; Immunoglobulin-like transcript 6; leucocyte immunoglobulin-like receptor; Leukocyte immunoglobulin-like receptor 4; leukocyte immunoglobulin-like receptor A3; leukocyte immunoglobulin-like receptor subfamily A member 3; leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member3; LILRA3; LIR4; LIR4CD85E; LIR-4HM43e3; Monocyte inhibitory receptor HM43/HM31

References

(1)Ordó?ez, D, Sánchez, A J, Martínez-Rodríguez, J E, et al. Multiple sclerosis associates with LILRA3 deletion in Spanish patients[J]. Genes & Immunity, 2009, 10(6):579-585.

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