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Biotinylated Human MAGE-A4 (HLA-A*02:01) Complex Protein

Product Info
Source

Recombinant Biotinylated Human MAGE-A4 (HLA-A*02:01) Complex Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and GVYDGREHTV peptide.[Accession | P04439&P61769&GVYDGREHTV]

Molecular Weight

The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

Storage

The product should be stored at -70℃. Please do not repeated freeze-thaw cycles.

Assay Data
Tris-Bis PAGE

Biotinylated Human MAGE-A4 (HLA-A*02:01) Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human MAGE-A4 (HLA-A*02:01) Complex was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Biotinylated Human MAGE-A4 (HLA-A*02:01) Complex at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-MAGE-A4 Antibody, hFc Tag with the EC50 9.2ng/ml determined by ELISA.

Background

Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) Complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and GVYDGREHTV peptide of  the MAGE-A4.

Synonyms

HLA0201; MHC I;MAGE-A4;Melanoma-associated antigen 4;MAGE-4 antigen;Cancer/testis antigen 1.4;CT1.4;MAGE-X2 antigen;MAGE-41 antigen

References

(1)Kageyama S , Ikeda H , Miyahara Y , et al. Adoptive Transfer of MAGE-A4 T-cell Receptor Gene-Transduced Lymphocytes in Patients with Recurrent Esophageal Cancer[J]. Clinical Cancer Research, 2015, 21(10):2268.

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