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Human GP100 (HLA-A*02:01) Complex Protein

Product Info
Source

Recombinant Human GP100 (HLA-A*02:01) Complex Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and YLEPGPVTA peptide.[Accession | P04439&P61769&YLEPGPVTA]

Molecular Weight

The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human GP100 (HLA-A*02:01) Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human GP100 (HLA-A*02:01) Complex was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human GP100 (HLA-A*02:01) Complex at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GP100 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 32.9ng/ml determined by ELISA.

Background

Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and YLEPGPVTA peptide of  the gp100.

Synonyms

HLA0201;MHC I;gp100;Glycoprotein 100;ME20-M;Melanocytes lineage-specific antigen GP100;P100

References

(1)Abad J D , Wrzensinski C , Overwijk W , et al. T-Cell Receptor Gene Therapy of Established Tumors in a Murine Melanoma Model[J]. Journal of Immunotherapy, 2008, 31(1):1-6.

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