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Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer Protein

Product Info
Source

Recombinant Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQC peptide.[Accession | P04439&P61769&SLLMWITQC]

Molecular Weight

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

Storage

The product should be stored at -70℃. Please do not repeated freeze-thaw cycles.

Assay Data
Tris-Bis PAGE

Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-NY-ESO-1(HLA-A*02:01) Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA.

SPR Data

Anti-hFc IgG capture Anti-NY-ESO-1 (HLA-A*02:01) Antibody, Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer as analyte; The affinity constant of 0.15 nM as determined in SPR assay (Biacore T200).

Background

NY-ESO-1 is a human tumor antigen of the cancer/testis family. It is highly expressed in many poor-prognosis melanomas.The Human HLA-A*0201 NY-ESO-1 (SLLMWITQC) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and SLLMWITQC peptide of  the NY-ESO-1 .

Synonyms

MHC;MY-ESO-1;Cancer/testis antigen 6.1;L antigen family member 2;CT6.1;LAGE-2

References

(1)Donnellan Z , Bossi G , Harper J , et al. ImmTACs: bi-specific TCR-anti-CD3 fusions for targeted tumour killing[J]. Journal for ImmunoTherapy of Cancer, 2015, 3(Suppl 2):P299.

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