Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein (MHC-HM406T)
Catalog: MHC-HM406T
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| Product Description | Recombinant Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide. |
Product Background |
HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes. |
Product Category |
Recombinant Protein |
Target |
HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer |
| Synonyms | HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1 |
| MHC Form | Tetramer |
| Allele | HLA-E*01:03 |
Gene/Protein ID |
P13747(HLA-E*01:03)&P61769(B2M)&VMAPRTLVL |
Species |
Human |
Exact Sequence |
Gly25-Ile305(HLA-E*01:03), Ile21-Met119(B2M) and VMAPRTLVL peptide |
| Molecular Weight | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 17.61 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human NKG2A&CD94, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag with an affinity constant of 17.61 nM as determined in SPR assay (Biacore T200) (QC Test). |
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Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag with an affinity constant of 1.65 uM as determined in SPR assay (Biacore T200). |
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The purity of Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer is greater than 95% as determined by SEC-HPLC. |
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Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%. |
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