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Human HLA-E Complex Tetramer Protein

Product Info
Source

Recombinant Human HLA-E Complex Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Ile305(HLA-E),Ile21-Met119(B2M) and VMAPRTLVL peptide .[Accession | P61769&P13747&VMAPRTLVL]

Molecular Weight

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

Storage

The product should be stored at -70℃. Please do not repeated freeze-thaw cycles.

Assay Data
Tris-Bis PAGE

Human HLA-E Complex Tetramer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human HLA-E Complex Tetramer is greater than 95% as determined by SEC-HPLC.

SPR Data

Human CD94&NKG2A, His Tag immobilized on CM5 Chip can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 0.30μM as determined in a SPR assay (Biacore T200).

Background

HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes.

Synonyms

HLA class I histocompatibility antigen, alpha chain E;HLAE;sHLA-E

References

(1) Kanevskiy L, Erokhina S, Kobyzeva P, Streltsova M, Sapozhnikov A, Kovalenko E. Dimorphism of HLA-E and its Disease Association. Int J Mol Sci. 2019 Nov 4;20(21):5496. doi: 10.3390/ijms20215496. PMID: 31690066; PMCID: PMC6862560.

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