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Human WT-1 (HLA-A*02:01) Complex Protein

Product Info
Source

Recombinant Human WT-1 (HLA-A*02:01) Complex Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide.[Accession | P04439&P61769&RMFPNAPYL]

Molecular Weight

The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human WT-1 (HLA-A*02:01) Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human WT-1 (HLA-A*02:01) Complex was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human WT-1 (HLA-A*02:01) Complex at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-WT-1 Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA.

SPR Data

Human WT-1 (HLA-A*02:01) Complex, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-WT-1 (HLA-1*02:01) Antibody, hFc Tag with an affinity constant of 0.33nM as determined in SPR assay (Biacore T200).

Background

The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A*0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A*0201 of  the MHC Class I, B2M and RMFPNAPYL peptide of  the WT-1.

Synonyms

MHC;WT1;RMF;Wilms tumor protein

References

(1)Dao T , Pankov D , Scott A , et al. Therapeutic bispecific T-cell engager antibody targeting the intracellular oncoprotein WT1[J]. Nature Biotechnology, 2015.

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