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Human MSLN/Mesothelin Protein

Product Info
Source

Recombinant Human MSLN/Mesothelin Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu296-Asp580.[Accession | Q13421-2]

Molecular Weight

The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human MSLN/Mesothelin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human MSLN/Mesothelin was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human MSLN,His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-MSLN Ab.,hFc Tag with the EC50 of 15.84ng/ml determined by ELISA.

Background

Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

Synonyms

MSLN; CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP;Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein

References

(1)Adusumilli P S , Cherkassky L , Villena-Vargas J , et al. Regional delivery of mesothelin-targeted CAR T cell therapy generates potent and long-lasting CD4-dependent tumor immunity[J]. Science Translational Medicine, 2014, 6(261):261ra151-261ra151.

(2)Morello A , Sadelain M , Adusumilli P S . Mesothelin-Targeted CARs: Driving T cells to Solid Tumors[J]. Cancer Discovery, 2015, 6(2).

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