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Human Nectin-2/CD112 Protein

Product Info
Source

Recombinant Human Nectin-2 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln32-Leu360.[Accession | Q92692]

Molecular Weight

The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human Nectin-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human Nectin-2 at 0.5μg/ml (100μl/Well) on plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.93μg/ml determined by ELISA.

Background

Nectins are a small family of Ca -independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions . They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins.Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Has some neurite outgrowth-promoting activity.

Synonyms

CD112 antigen; CD112; Herpes virus entry mediator B; Herpesvirus entry mediator B; herpesvirus entry protein B; HVEB; HVEBpoliovirus receptor-like 2; MPH; nectin 2; Nectin2; Nectin-2; poliovirus receptor-related 2 (herpesvirus entry mediator B); poliovirus receptor-related protein 2; PRR2; PRR2nectin-2; PVRL2; PVRR2poliovirus receptor related 2

References

(1)Oshima T , Sato S , Kato J , et al. Nectin-2 is a potential target for antibody therapy of breast and ovarian cancers[J]. Molecular Cancer, 2013, 12(1):60.

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