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Human OX40/TNFRSF4/CD134 Protein

Product Info
Source

Recombinant Human OX40/TNFRSF4/CD134 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu29-Ala216.[Accession | P43489]

Molecular Weight

The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human OX40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human OX40 is greater than 95% as determined by SEC HPLC.

Background

Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.

Synonyms

CD134 antigen; CD134;OX40; OX40L receptor; TNFRSF4;ACT-135; ACT35 antigen; ACT35ATC35 antigen; Ly-70; OX40 cell surface antigen; OX40 homologue;  OX40lymphoid activation antigene ACT35; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; Txgp1; TXGP1L; TXGP1LOX40 antigen

References

(1)Al-Shamkhani A , Mallett S , Brown M H , et al. Affinity and Kinetics of the Interaction between Soluble Trimeric OX40 Ligand, a Member of the Tumor Necrosis Factor Superfamily, and Its Receptor OX40 on Activated T Cells[J]. Journal of Biological Chemistry, 1997, 272(8):5275-5282.

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