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Human PD-1/PDCD1 Protein

Product Info
Source

Recombinant Human PD-1/PDCD1 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Leu25-Gln167.[Accession | Q8IX89]

Molecular Weight

The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 35-55 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human PD-1 on Tris-Bis PAGE under reduced . The purity is greater than 95%.

SEC-HPLC

The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human PD-1 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA.

Batch to Batch Consistency

Immobilized Human PD-1 at 0.5μg/ml (100μl/Well). Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 45.0/41.6/45.7ng/ml determined by ELISA.

Background

Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.

Synonyms

Programmed cell death protein 1;Protein PD-1;PDCD1;PD1

References

(1)Blank C , Mackensen A . Contribution of the PD-L1/PD-1 pathway to T-cell exhaustion: an update on implications for chronic infections and tumor evasion[J]. Cancer Immunology Immunotherapy, 2007, 56(5):739-745.

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