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Human PD-L1/B7-H1 Protein

Product Info
Source

Recombinant Human PD-L1/B7-H1 Protein is expressed from Expi293 with His tag at the C-terminal. It contains Phe19-Arg238.[Accession | Q9NZQ7]

Molecular Weight

The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 35-42 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human PD-L1 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human PD-L1, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-PD-L1 Ab., hFc Tag with the EC50 of 6.1ng/ml determined by ELISA.

Batch to Batch Consistency

Immobilized Human PD-L1, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-PD-L1 Ab., hFc Tag with the EC50 of 6.1/6.4/6.2ng/ml determined by ELISA.

37℃ Stability Test

Immobilized Human PD-L1, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-PD-L1 Ab., hFc Tag with the EC50 of 6.4/6.4/6.5/6.6ng/ml determined by ELISA.

Background

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

Synonyms

CD274 antigenMGC142294; CD274 molecule; CD274;PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; B7H1PDCD1L1;  PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296;  PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1; PDL1

References

(1)Blank C , Mackensen A . Contribution of the PD-L1/PD-1 pathway to T-cell exhaustion: an update on implications for chronic infections and tumor evasion[J]. Cancer Immunology Immunotherapy, 2007, 56(5):739-745.

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