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Human PSMA/FOLH1 Protein

Product Info
Source

Recombinant Human PSMA/FOLH1 Protein is expressed from Expi293 with His tag at the N-terminal. It contains Lys44-Ala750.[Accession | Q04609]

Molecular Weight

The protein has a predicted MW of 80.6 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human PSMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human PSMA is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human PSMA at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA.

Background

Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.

Synonyms

FOLH1; GCP2; PSMA; PSMAFGCP; Cell growth-inhibiting gene 27 protein; cell growth-inhibiting protein 27; EC 3.4.17.21; FGCP; folate hydrolase (prostate-specific membrane antigen) 1; Folate hydrolase 1; FOLHNAALADase I; Folylpoly-gamma-glutamate carboxypeptidase; GCPII; GCPIImGCP; glutamate carboxylase II; glutamate carboxypeptidase 2; Glutamate carboxypeptidase II; Membrane glutamate carboxypeptidase; mopsm; NAALAD1; NAALAD1N-acetylated-alpha-linked acidic dipeptidase I; NAALADase I; NAALAdase; N-acetylated alpha-linked acidic dipeptidase 1; prostate specific membrane antigen variant F; Prostate-specific membrane antigen;PSMPteroylpoly-gamma-glutamate carboxypeptidase

References

(1)Song C , Francesca Z , Williams D G , et al. Antitumor Activity of MEDI3726 (ADCT-401), a Pyrrolobenzodiazepine Antibody-drug Conjugate Targeting PSMA, in Pre-clinical Models of Prostate Cancer[J]. Molecular Cancer Therapeutics, 2018:molcanther.0982.2017-.

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