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Human PVRIG Protein

Product Info
Source

Recombinant Human PVRIG Protein is expressed from Expi293 with mFc tag at the C-terminal. It contains Thr41-Asp171 .[Accession | Q6DKI7-1]

Molecular Weight

The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human PVRIG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human PVRIG is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human PVRIG at 1μg/ml (100μl/Well) on plate.Dose response curve for Anti-PVRIG Ab., hFc Tag with the EC50 of 13.8ng/ml determined by ELISA.

Background

Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family.PVRIG is a cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.

Synonyms

C7orf15; C7orf15MGC138295; CD112R; MGC104322; MGC138297; MGC2463; poliovirus receptor related immunoglobulin domain containing; Poliovirus receptor-related immunoglobulin domain-containing protein; PVRIG; transmembrane protein PVRIG

References

(1)PVRIG and PVRL2 Are Induced in Cancer and Inhibit CD8 T-cell Function. 2019.

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