Human PVRIG Protein (PVR-HM301)

  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PVRIG, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PVRIG Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. The affinity constant of 0.349 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Thr41-Asp171

Catalog: PVR-HM301

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Product Description Recombinant Human PVRIG Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Thr41-Asp171.

Product Background

Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family.PVRIG is a cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.

Product Category

Recombinant Protein

Target

PVRIG
Synonyms C7orf15; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1

Gene/Protein ID

Q6DKI7-1

Species

Human

Exact Sequence

Thr41-Asp171
Molecular Weight The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human PVRIG, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PVRIG Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. The affinity constant of 0.349 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-mFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PVRIG, mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human Nectin-2, His Tag with an affinity constant of 0.349 uM as determined in SPR assay (Biacore T200).
Immobilized Human PVRIG, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-PVRIG Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
Immobilized Human Nectin-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.22ug/ml determined by ELISA (QC Test).
The purity of Human PVRIG is greater than 95% as determined by SEC-HPLC.
Human PVRIG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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